APA
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Muñoz-Barrios, A., Sopeña-Torres, S., Ramos, B., López, G., del Hierro, I., Díaz-González, S., … Molina, A. (2020). Differential expression of fungal genes determines the lifestyle of Plectosphaerella strains during Arabidopsis thaliana colonization. Molecular Plant-Microbe Interactions : MPMI.
Chicago/Turabian
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Muñoz-Barrios, Antonio, Sara Sopeña-Torres, B. Ramos, G. López, Irene del Hierro, Sandra Díaz-González, P. González-Melendi, et al. “Differential Expression of Fungal Genes Determines the Lifestyle of Plectosphaerella Strains during Arabidopsis Thaliana Colonization.” Molecular plant-microbe interactions : MPMI (2020).
MLA
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Muñoz-Barrios, Antonio, et al. “Differential Expression of Fungal Genes Determines the Lifestyle of Plectosphaerella Strains during Arabidopsis Thaliana Colonization.” Molecular Plant-Microbe Interactions : MPMI, 2020.
BibTeX Click to copy
@article{antonio2020a,
title = {Differential expression of fungal genes determines the lifestyle of Plectosphaerella strains during Arabidopsis thaliana colonization.},
year = {2020},
journal = {Molecular plant-microbe interactions : MPMI},
author = {Muñoz-Barrios, Antonio and Sopeña-Torres, Sara and Ramos, B. and López, G. and del Hierro, Irene and Díaz-González, Sandra and González-Melendi, P. and Mélida, Hugo and Fernández-Calleja, Vanessa and Mixão, V. and Martín-Dacal, Marina and Marcet-Houben, M. and Gabaldón, T. and Sacristán, S. and Molina, A.}
}
The fungal genus Plectosphaerella comprises species and strains with different lifestyles on plants, such as P. cucumerina, which has served as model for the characterization of Arabidopsis thaliana basal and non-host resistance to necrotrophic fungi. We have sequenced, annotated and compared the genomes and transcriptomes of three Plectosphaerella strains with different lifestyles on A. thaliana: PcBMM, a natural pathogen of wild-type plants (Col-0); Pc2127, a non-pathogenic strain on Col-0 but pathogenic on the immunocompromised cyp79B2 cyp79B3 mutant; and P0831 strain, that was isolated from a natural population of A. thaliana and is shown here to be non-pathogenic and to grow epiphytically on Col-0 and cyp79B2 cyp79B3 plants. The genomes of these Plectosphaerella strains are very similar and do not differ in the number of genes with pathogenesis-related functions, with the exception of secreted Carbohydrate-Active Enzymes (CAZymes) that are up to five times more abundant in the pathogenic strain PcBMM. Analysis of the fungal transcriptomes in inoculated Col-0 and cyp79B2 cyp79B3 plants at initial colonization stages confirm the key role of secreted CAZymes in the necrotrophic interaction, since PcBMM expresses more genes encoding secreted CAZymes than Pc2127 and P0831. We also show that P0831 epiphytic growth on A. thaliana involves the transcription of specific repertoires of fungal genes, that might be necessary for epiphytic growth adaptation. Overall these results suggest that in planta expression of specific sets of fungal genes at early stages of colonization determine the diverse lifestyles and pathogenicity of Plectosphaerella strains.