Journal article
S. Sevillano, R. Cobos, P. García-Angulo, A. Alonso-Monroy, M. Alvarez-Rodríguez, José Manuel Álvarez-Pérez, M. A. Olego, J. Acebes, E. Garzón, J. Coque
2014
2014
Semantic Scholar
DOI
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APA
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Sevillano, S., Cobos, R., García-Angulo, P., Alonso-Monroy, A., Alvarez-Rodríguez, M., Álvarez-Pérez, J. M., … Coque, J. (2014). Manganese transporter protein MntH is required for virulence of Xylophilus ampelinus, the causal agent of bacterial necrosis in grapevine.
Chicago/Turabian
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Sevillano, S., R. Cobos, P. García-Angulo, A. Alonso-Monroy, M. Alvarez-Rodríguez, José Manuel Álvarez-Pérez, M. A. Olego, J. Acebes, E. Garzón, and J. Coque. “Manganese Transporter Protein MntH Is Required for Virulence of Xylophilus Ampelinus, the Causal Agent of Bacterial Necrosis in Grapevine” (2014).
MLA
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Sevillano, S., et al. Manganese Transporter Protein MntH Is Required for Virulence of Xylophilus Ampelinus, the Causal Agent of Bacterial Necrosis in Grapevine. 2014.
BibTeX Click to copy
@article{s2014a,
title = {Manganese transporter protein MntH is required for virulence of Xylophilus ampelinus, the causal agent of bacterial necrosis in grapevine},
year = {2014},
author = {Sevillano, S. and Cobos, R. and García-Angulo, P. and Alonso-Monroy, A. and Alvarez-Rodríguez, M. and Álvarez-Pérez, José Manuel and Olego, M. A. and Acebes, J. and Garzón, E. and Coque, J.}
}
Abstract
Background and Aims
The aim of this study is to identify proteins involved in the pathogenicity/virulence of Xylophilus ampelinus. Characterisation of these proteins could provide new insights into putative targets for controlling bacterial necrosis in grapevines.
Methods and Results
Transposon insertion mutagenesis was used to isolate X. ampelinus mutants exhibiting an altered virulence. Characterisation of one of the avirulent mutants revealed the insertion of a transposon into the mntH gene encoding the major manganese transporter. Virulence tests on grapevine leaves clearly showed that the virulence of these mutants was significantly reduced. Phenotypic analysis of an mntH mutant indicated that the MntH protein is a Mn++ transporter but that MntH does not play a significant role in the transport of Fe++ or Cu++. The MntH mutants exhibited an increased sensitivity to hydrogen peroxide, although catalase and superoxide dismutase activities were not significantly affected.
Conclusion
The MntH protein plays a significant role in the virulence of X. ampelinus.
Significance of the Study
This is the first report showing that transposon mutagenesis is an effective strategy for the isolation of X. ampelinus mutants. It is also the first report characterising a gene encoding a protein involved in virulence in this grapevine pathogen.